Separation of haemoglobins on cellulose acetate.

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Rapid electrophoresis and quantitation of haemoglobins on cellulose acetate.

A rapid and reproducible electrophoretic method for the separation and quantitation of haemoglobins on cellulose acetate is described. The accuracy of the method and its possible sources of error are discussed. The normal range for haemoglobin A(2) by this method is 1% to 3% of the total haemoglobin concentration. Blood samples from 32 thalassaemic patients showed haemoglobin A(2) values of 3.5...

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Carbon dioxide (CO2) release from the exhaust gas streams of power plants that burn fossil fuels contributes the most emission of this gas, which includes more than half of the greenhouse effects. Thus, the separation of CO2 from exhaust gases is one of the main environmental concerns. Membrane technology, due to many advantages, has attracted many research attentions among the various gas sepa...

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The Staining of Lactic Dehydrogenase Isoenzymes after Electrophoretic Separation on Cellulose Acetate.

Established electrophoretic techniques for the separation of the lactate dehydrogenase (L.D.H.) isoenzymes of tissues and body fluids, although of value in clinical medicine, are too elaborate for most routine laboratories. A simple method for the separation of these isoenzymes on cellulose acetate and their histochemical staining is described. Spectrophotometric estimations of the total L.D.H....

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Zinc acetate as a precipitant of unstable haemoglobins.

Whereas the addition of four moles of zinc acetate to one of normal human haemoglobin at pH 7.4 results in a clear solution, addition to unstable haemoglobin results in precipitate formation. Within certain limits no false-positive results are obtained with methaemoglobin, and with fetal haemoglobin. With excess zinc, normal haemoglobin also precipitates, but this process is reversible and can ...

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ژورنال

عنوان ژورنال: Journal of Clinical Pathology

سال: 1969

ISSN: 0021-9746

DOI: 10.1136/jcp.22.1.109